Exploring GIs in Yeast:
Identifying Differential Essential Genes and Mapping Genetic Interaction Networks in a Set of Wild Yeast Isolates
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Guihong Tan, Thiago Fernandes, Fang He
An individual's genome, termed genetic background, is unique due to the distinct collection of variants across all chromosomes. The biological consequence of this variation on our traits is still unknown. Previously, our lab has shown that gene essentiality and signaling network structure can be different in two closely related S. cerevisiae genetic backgrounds (S288c vs. Σ1278b). To understand the spectrum of change within a single species, we have used CRISPR-Cas9 to construct seven genome-wide deletion collections in diverse yeast backgrounds. With this, we are surveying gene essentiality change within a species. Additionally, these collections have been integrated into the Synthetic Genetic Array (SGA) pipeline to study the robustness of the genetic interaction network to differences in genetic background. This project will further our understanding of phenotype and network dynamics due to natural variation.
