Project Links
Exploring GIs in a Human Cell Line:
Mapping Genetic Interactions of Human Essential Genes
This project is a collaboration with the lab of Jason Moffat, Genetics and Genome Biology at Sick Kids
People
Zi Yang Wang, Ermira Shuteriqi, Wendy Liang, Ryan Climie, Lyudmila Nedyalkova, Matej Usaj
In yeast, we found that hypomorphic alleles of essential genes have substantially more GIs than deletions of non-essential genes. We are using several approaches to systematically create hypomorphic alleles of human essential genes and cataloguing a collection of stable cell lines. These are then used as queries to screen for genetic interactions via a genome-wide CRISPR-Cas9 knockout method.
