Chemical Genetics:
Functional Characterization of Chemical-Genetic Interactions
People
Urvi Bhojoo, Matej Usaj
We are developing a high-throughput, human cell-based platform for chemical genetic profiling of drugs and novel bioactive compounds using CRIPSPR technology. In collaboration with interdisciplinary research groups, we have generated a unique dataset of ~150 compounds that reveal how drugs work at the genetic level in human cells. These efforts have led to the identification of 22 previously uncharacterized small molecules with promising therapeutic potential from the RIKEN natural product library. Furthermore, with further experimentation and analysis, we uncovered the critical molecular pathway for the cytotoxicity of a novel compound and devised a sensitization strategy that can be exploited in cancer treatment. This work is not only expanding our understanding of the interactions between drugs and our genome, but also hold promise for the development of targeted therapies for various diseases.
