Systematic Analysis of Complex Genetic Interactions

In this study 182 double mutants and 364 corresponding single mutant control strains were screened for genetic interactions against a diagnostic array of ~1,200 strains. Every double mutant query strain was screened alongside its two single mutant control strains in two independent replicates for a total of 1,092 screens. We tested 410,399 double and 195,666 triple mutants for fitness defects and identified 9,363 digenic and 3,196 negative trigenic interactions. The genetic interactions displayed here represent digenic interactions of single mutant control query strains and final trigenic interaction scores adjusted for digenic interactions. We suggest to use an established interaction magnitude cut-off for digenic interactions (p < 0.05, |ε| > 0.08) and trigenic interactions (p < 0.05, τ < -0.08).